How to Prepare
You will need:
- Insulin Syringe
- Bacteriostatic or Sterile Water
- Alcohol Wipes
What is Peptide Reconstitution?
In its most basic form reconstitution is the practice of restoring a dried substance to its original state by the addition of water.
So the plan is to simply prepare your lyophilized Peptides into an injectable solution.
Sterile Water versus Bacteriostatic Water
Both are essentially water which is free from any microbes or bacteria. Both are also fine for subcutaneous injection and the only real difference between the two is that bacteriostatic water has an additional anti-bacterial agent in the form of benzyl alcohol.
Reconstituting your Peptides
When you're ready to prepare your peptides you'll need good light in addition to a clean and tidy workspace.
The first step is to thoroughly clean your hands with a sterile hand wash. We've all seen doctors and health care professionals at work and there's a reason they clean their hands before dealing with needles.
Next, prepare the vials.
Begin by removing the top cap and exposing the rubber stopper. Prepare both the water and the peptides at the same time.
With an antiseptic wipe, clean the tops of both vials.
After this prepare your syringe.
To do this, take the caps off, exposing both the needle and the plunger.
Now you're ready to reconstitute your peptides.
You'll do this by mixing the correct volume of water with the peptides.
Pierce the rubber lid of the bacteriostatic water with your syringe and turn the vial of water and syringe upside down. This ensures you'll only draw up water; no air. If using an ampoule of water, break the lid off and carefully insert the needle into the ampoule.
Pull the plunger and withdraw the required amount of water into the syringe.
Then, use the syringe to pierce the peptide vial and gently inject the water into the vial. This process is critical, keep it slow as too much swirling or shaking of the water can cause damage to the peptides.
As soon as the water enters the vial the reconstitution should begin and you'll start to see the peptides dissolve. Do not shake the vial.
Next, to equalise the pressure in the vial you'll want to vent it. To do this take the syringe out and remove the plunger completely. This should give you a straw-like tool. Then re-pierce the vial and this should remove any imbalances in pressure.
Make sure the peptide has completely dissolved and that there is no powder left in the vial.
If any powder does remain, gently turn the vial over and over until there is no trace of powder.
After reconstituting, store the vial(s) in the fridge.
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